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Comparative Study
. 2005 Feb;43(2):892-5.
doi: 10.1128/JCM.43.2.892-895.2005.

Mycobacterium marinum infections in fish and humans in Israel

M Ucko  1 , A Colorni
Affiliations
Comparative Study

Mycobacterium marinum infections in fish and humans in Israel

M Ucko et al. J Clin Microbiol. 2005 Feb.

Abstract

Israeli Mycobacterium marinum isolates from humans and fish were compared by direct sequencing of the 16S rRNA and hsp65 genes, restriction mapping, and amplified fragment length polymorphism analysis. Significant molecular differences separated all clinical isolates from the piscine isolates, ruling out the local aquaculture industry as the source of human infections.

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Figures

FIG. 1.
FIG. 1.
Enzyme restriction mapping. A 614-bp product from the 16S rRNA gene was cleaved with the restriction enzyme MslI. Lanes: M, 100-bp molecular size marker; 1 to 3, Israeli M. marinum clinical isolates; 4, M. marinum ATCC 927; 5, M. marinum isolate S4, from a snakehead (C. striata); 6, M. marinum Cyprinum CC240299 (GenBank accession no. AF456239); 7, M. marinum Eilaticum DL240490 (GenBank accession no. AF456238).
FIG. 2.
FIG. 2.
Distance tree derived from UPGMA cluster analysis of the AFLP patterns of all strains used in this study. Numbers on the branches indicate bootstrap proportions (1,000 replicates); only bootstrap values above 70% are displayed on the tree. Mycobacterium sp. strain DL049 was used as an outgroup.
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