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. 2005 Feb;43(2):925-7.
doi: 10.1128/JCM.43.2.925-927.2005.

Quality control guidelines for testing gram-negative control strains with polymyxin B and colistin (polymyxin E) by standardized methods

Ronald N Jones  1 , Tamara R Anderegg, Jana M Swenson; Quality Control Working Group
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Quality control guidelines for testing gram-negative control strains with polymyxin B and colistin (polymyxin E) by standardized methods

Ronald N Jones et al. J Clin Microbiol. 2005 Feb.

Abstract

An eight-laboratory study addressed the urgent need for quality control (QC) ranges for susceptibility determination when testing colistin (polymyxin E) and polymyxin B, two polycationic peptide antimicrobial agents, against multidrug-resistant gram-negative bacilli. For Escherichia coli ATCC 25922l, the QC ranges were as follows: for colistin, 0.25 to 1 microg/ml (11 to 17 mm), and for polymyxin B, 0.25 to 2 microg/ml (13 to 19 mm). For Pseudomonas aeruginosa ATCC 27853, the QC ranges were as follows: for colistin, 0.25 to 2 microg/ml (11 to 17 mm), and for polymyxin B, 0.25 to 2 mug/ml (14 to 18 mm). More than 97% of all reported QC results were within these proposed ranges.

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