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. 2005 Feb;43(2):931-2.
doi: 10.1128/JCM.43.2.931-932.2005.

Use of the restriction enzyme EcoRI for pulsed-field gel electrophoretic analysis of Helicobacter pylori

Yoshio Hosaka  1 , Kazuhiko Irinoda, Ryuichi Nakano, Satosi Tanabe, Wasaburo Koizumi, Katunori Saigenji, Matsuhisa Inoue
Affiliations

Use of the restriction enzyme EcoRI for pulsed-field gel electrophoretic analysis of Helicobacter pylori

Yoshio Hosaka et al. J Clin Microbiol. 2005 Feb.

Abstract

Pulsed-field gel electrophoretic (PFGE) analysis of Helicobacter pylori isolates is not commonly employed because of the inability to compare the typing with other typing systems. We adapted the PFGE analysis for H. pylori by using EcoRI and slightly modified our laboratory methods to improve the typing of isolates (typeability was 97%).

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Figures

FIG. 1.
FIG. 1.
(A and B) PFGE analysis of H. pylori with the use of five restriction enzymes to cut the chromosomal DNA (EcoRI, HindIII, NotI, Sau3A, and SmaI). (C) Analysis by our modified laboratory methods. Lanes M, contour-clamped homogeneous electric field DNA size standards used as PFGE size standards.
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