Duplex PCR to differentiate between Mycoplasma synoviae and Mycoplasma gallisepticum on the basis of conserved species-specific sequences of their hemagglutinin genes
- PMID: 15695715
- PMCID: PMC548031
- DOI: 10.1128/JCM.43.2.948-950.2005
Duplex PCR to differentiate between Mycoplasma synoviae and Mycoplasma gallisepticum on the basis of conserved species-specific sequences of their hemagglutinin genes
Abstract
We developed a duplex PCR assay targeting the hemagglutinin multigene families, vlhA and pMGA, of Mycoplasma synoviae and Mycoplasma gallisepticum, respectively. The assay proved to be specific and sensitive enough to justify its use for the simultaneous detection of the two major avian mycoplasma species from field isolates.
Figures


References
-
- Ben Abdelmoumen, B., and R. S. Roy. 1995. Antigenic relatedness between seven avian mycoplasma species as revealed by Western blot analysis. Avian Dis. 39:250-262. - PubMed
-
- Ben Abdelmoumen, B., and R. S. Roy. 1995. An enzyme-linked immunosorbent assay for detection of avian mycoplasmas in culture. Avian Dis. 39:85-93. - PubMed
-
- Ben Abdelmoumen, B., R. S. Roy, and R. Brousseau. 1999. Cloning of Mycoplasma synoviae genes encoding specific antigens and their use as species-specific DNA probes. J. Vet. Diagn. Investig. 11:162-169. - PubMed
-
- Bradbury, J. M., and F. T. W. Jordan. 1971. The adsorption of gamma globulins to Mycoplasma gallisepticum and the possible role in nonspecific serological reactions. Vet. Rec. 89:318. - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources