[Relationship of the genetic diversity within the V-region, P-region and C-terminus of surface protein of Streptococcus mutans to dental caries]
- PMID: 15702767
[Relationship of the genetic diversity within the V-region, P-region and C-terminus of surface protein of Streptococcus mutans to dental caries]
Abstract
Objective: To gain an understanding of the relationship between the genetic diversity within the V-region, P-region, C-terminus of surface protein of Streptococcus mutans (serotype c) and their adherent abilities.
Methods: The clinical isolates of S. mutans (serotype c) in two groups with different adherent abilities (cpm>2000, cpm<1000) had been prepared in our experimental laboratory. The genome DNA was extracted, and the spaP-pv (2060-3157 bp) and spaP-c (4003-4851 bp) were amplified by PCR, respectively. Genetic diversity was assessed by restriction fragment-length polymorphism (RFLP) with restriction endonucleases Alu I.
Results: (1) Two different patterns of spaP-pv RFLP among strains were revealed after the amplified product being digested with Alu I. The distributions of two genotypes (a and b) in the clinical strains with different adherent abilities differed significantly (P<0.05). The proportion of genotype b in the strains with higher adherent abilities was higher than that in the strains with lower adherent abilities. (2) Two different patterns (c and d) of spaP-c RFLP among strains were also revealed after the spaP-c being digested with Alu I. No statistically significant difference was observed in the distribution of the two genotypes in the clinical strains.
Conclusion: The genetic diversity within spaP-pv of clinical isolates of S. mutans might be related to the differences of their adherent abilities.
Similar articles
-
[A study of genetic diversity in lactate dehydrogenase of Streptococcus mutans from clinical isolates].Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Sep;37(5):781-4. Sichuan Da Xue Xue Bao Yi Xue Ban. 2006. PMID: 17037751 Chinese.
-
[Genetic diversity of F-ATPase subunits gene uncA amplified from Streptococcus mutans clinical isolates].Zhonghua Kou Qiang Yi Xue Za Zhi. 2006 Jan;41(1):29-32. Zhonghua Kou Qiang Yi Xue Za Zhi. 2006. PMID: 16620624 Chinese.
-
Characterization of Streptococcus mutans diversity by determining restriction fragment-length polymorphisms of the gtfB gene of isolates from 5-year-old children and their mothers.Antonie Van Leeuwenhoek. 2005 Jul;88(1):75-85. doi: 10.1007/s10482-005-1674-3. Antonie Van Leeuwenhoek. 2005. PMID: 15928979
-
[Evaluation of cariogenic potential of Streptococcus mutans isolated from caries-free and -active persons: adherence properties to saliva-coated hydroxyapatite].Hua Xi Kou Qiang Yi Xue Za Zhi. 2000 Dec;18(6):416-8. Hua Xi Kou Qiang Yi Xue Za Zhi. 2000. PMID: 12539475 Chinese.
-
[Genetic diversity of ATP synthase cab subunits amplified from Streptococcus mutans clinical isolates from Uyghur children with different caries susceptibility].Zhonghua Kou Qiang Yi Xue Za Zhi. 2012 Sep;47(9):547-51. doi: 10.3760/cma.j.issn.1002-0098.2012.09.010. Zhonghua Kou Qiang Yi Xue Za Zhi. 2012. PMID: 23141730 Chinese.
Publication types
MeSH terms
Substances
LinkOut - more resources
Medical