doi: 10.1016/j.femsim.2004.09.002.
A study on the bacterial photo-toxicity of phenothiazinium based photosensitisers
Affiliations
- PMID: 15708310
- DOI: 10.1016/j.femsim.2004.09.002
Item in Clipboard
A study on the bacterial photo-toxicity of phenothiazinium based photosensitisers
FEMS Immunol Med Microbiol.
.
Free article
Abstract
"Comet assay" showed light activated (3.15 Jcm-2 over 30 min) phenothiazinium based photosensitisers (PhBPs) to induce photo-damage of Staphylococcus aureus DNA, as indicated by DNA "tails" between 80 and 120 microm. In general, PhBPs exhibited significant singlet oxygen yields (Phi(DeltaPhBP)>0.7), suggesting the use of type II mechanisms of photo-oxidation. However, the photodynamic action of PhBPs on DNA showed generally insignificant production of 7,8-dihydro-8-oxo-2'-deoxyguanosine, normally a major product of type II DNA photo-oxidation. These combined results show DNA to be a major site of action of PhBPs and suggest that this action may involve type II attack on a nucleoside(s) other than guanosine.
Similar articles
-
Different mechanisms for the photoinduced production of oxidative DNA damage by fluoroquinolones differing in photostability.Chem Res Toxicol. 1999 Sep;12(9):809-15. doi: 10.1021/tx980224j. Chem Res Toxicol. 1999. PMID: 10490502
-
Supramolecular cationic tetraruthenated porphyrin and light-induced decomposition of 2'-deoxyguanosine predominantly via a singlet oxygen-mediated mechanism.Photochem Photobiol. 1998 Nov;68(5):698-702. Photochem Photobiol. 1998. PMID: 9825700
-
Guanine-specific DNA damage photosensitized by the dihydroxo(tetraphenylporphyrinato)antimony(V) complex.J Photochem Photobiol B. 2006 Jan 2;82(1):37-44. doi: 10.1016/j.jphotobiol.2005.08.008. Epub 2005 Oct 17. J Photochem Photobiol B. 2006. PMID: 16230021
-
Phenothiazinium based photosensitisers--photodynamic agents with a multiplicity of cellular targets and clinical applications.Curr Drug Targets. 2005 Aug;6(5):615-27. doi: 10.2174/1389450054545962. Curr Drug Targets. 2005. PMID: 16026282 Review.
-
Phenothiazinium derivatives for pathogen inactivation in blood products.J Photochem Photobiol B. 2007 Jan 3;86(1):45-58. doi: 10.1016/j.jphotobiol.2006.07.005. Epub 2006 Sep 18. J Photochem Photobiol B. 2007. PMID: 16979899 Review.
Cited by
-
Bacteriophytochrome controls carotenoid-independent response to photodynamic stress in a non-photosynthetic rhizobacterium, Azospirillum brasilense Sp7.Sci Rep. 2012;2:872. doi: 10.1038/srep00872. Epub 2012 Nov 19. Sci Rep. 2012. PMID: 23173079 Free PMC article.
-
Escherichia coli Sterilization Using a Light-Emitting Diode and Methylene Blue.Laser Ther. 2019 Jun 30;28(2):97-102. doi: 10.5978/islsm.19-OR-05. Laser Ther. 2019. PMID: 32921907 Free PMC article.
-
In Vitro Effect of Toluidine Blue Antimicrobial Photodynamic Chemotherapy on Staphylococcus epidermidis and Staphylococcus aureus Isolated from Ocular Surface Infection.Transl Vis Sci Technol. 2019 Jun 21;8(3):45. doi: 10.1167/tvst.8.3.45. eCollection 2019 May. Transl Vis Sci Technol. 2019. PMID: 31259090 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
