[The relationship between the fragment deletion of mtDNA in old rat and the sensitivity to aminoglycoside antibiotic induced deafness]
- PMID: 15715416
[The relationship between the fragment deletion of mtDNA in old rat and the sensitivity to aminoglycoside antibiotic induced deafness]
Abstract
Objective: To analyze the relationship between the fragnent deletion of mitochondrial DNA (mtDNA) of inner ear in rats and aminoglycoside antibiotic induced deafness (AAID), and to investigate the possible role of mtDNA deletion in the sensitivity to aminoglycoside antibiotic induced deafness in old rat.
Method: Thirty wistar rats were divided into group A (old rat, > 24 months, n = 15) and group B (young rat, 4 months, n = 15) according to the age. Kanamycin (KM) was intraperitoneally injected 500 mg/kg per day for 10 consecutive day in all rats. The threshold of the auditory brainstem response (ABR) was measured before and after the drug administrations. The inner ear tissue was harvested and the mtDNA was amplified to identify the 4834 bp deletion by PCR technique.
Result: The elevation of the mean ABR thresholds in group A (42.08 +/- 8.19) dB peSPL was significantly higher than that in group B (12.71 +/- 4.42) dB peSPL (P < 0.01). In group A, 10 of the 15 rats demonstrate the mtDNA4834 deletion and the elevation of the mean ABR thresholds in the rats with mtDNA4834 deletion (45.00 +/- 7.67) dB peSPL was significantly higher than that without so deletion (33.33 +/- 4.08) dB peSPL (P < 0.01). However, mtDNA4834 deletion was negative in group B.
Conclusion: Deletion of mtDNA of inner ear tissue in old rat is found. The mtDNA deletion may play an important role in the hypersensitivity to aminoglycoside antibiotic ototoxiciy in old rats.
Similar articles
-
[The role of mtDNA deletion in the sensitivity to aminoglycoside antibiotic induced deafness].Zhonghua Er Bi Yan Hou Ke Za Zhi. 2000 Apr;35(2):94-7. Zhonghua Er Bi Yan Hou Ke Za Zhi. 2000. PMID: 12768661 Chinese.
-
[Mitochondrial DNA large deletions associated with presbycusis].Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2003 Nov;17(11):678-80. Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2003. PMID: 14971208 Chinese.
-
The effect of the mtDNA4834 deletion on hearing.Biochem Biophys Res Commun. 2006 May 26;344(1):425-30. doi: 10.1016/j.bbrc.2006.03.060. Epub 2006 Mar 24. Biochem Biophys Res Commun. 2006. PMID: 16616000
-
[The study of the high sensitivity to noise trauma in the inner ear mimetic aging model].Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2010 Oct;24(19):895-8, 900. Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2010. PMID: 21174752 Chinese.
-
Effects of dietary restriction and antioxidants on presbyacusis.Laryngoscope. 2000 May;110(5 Pt 1):727-38. doi: 10.1097/00005537-200005000-00003. Laryngoscope. 2000. PMID: 10807352 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Medical
Miscellaneous