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. 2005 May;61(2):245-50.
doi: 10.1016/j.mimet.2004.12.004. Epub 2004 Dec 30.

Measurement of limestone biodeterioration using the Ca2+ binding fluorochrome Rhod-5N

C J McNamara  1 , T D Perry 4th, K Bearce, G Hernandez-Duque, R Mitchell
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Measurement of limestone biodeterioration using the Ca2+ binding fluorochrome Rhod-5N

C J McNamara et al. J Microbiol Methods. 2005 May.

Abstract

Limestone and marble have been used extensively in the construction of modern and historic buildings. Microbial colonization and growth on these stone structures is common. Microbial deterioration of stone has been assessed by measuring Ca2+ released from the stone, using ion selective electrodes and titration with EDTA. In this study, the calcium binding fluorochrome Rhod-5N was used to measure Ca2+ released from limestone by endolithic bacteria as an indicator of biodeterioration. In a 17 d flask experiment, Ca2+ released by endolithic bacteria was twice that of uninoculated controls. Rhod-5N is a rapid and accurate method for measuring microbial biodeterioration of stone.

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