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. 2005 Mar;241(3):534-40.
doi: 10.1097/01.sla.0000154281.79639.89.

Prevention of intraperitoneal adhesions and abscesses by polysaccharides isolated from Phellinus spp in a rat peritonitis model

Jae-Sung Bae  1 , So-Jeo Ahn, Hyunee Yim, Kwang-Ho Jang, Hee Kyung Jin
Affiliations

Prevention of intraperitoneal adhesions and abscesses by polysaccharides isolated from Phellinus spp in a rat peritonitis model

Jae-Sung Bae et al. Ann Surg. 2005 Mar.

Abstract

Objective: To assess whether polysaccharides isolated from fungi, Phellinus spp, could reduce the adhesion and abscess formation in a rat peritonitis model.

Summary background data: Although polysaccharides from Phellinus spp is a well-known material with antiinflammatory properties, little is known regarding its ability to prevent intraperitoneal adhesions. We have assessed the adhesion- and abscess-reducing effect of polysaccharides from Phellinus gilvus (PG) and Phellinus linteus (PL) in a rat peritonitis model.

Methods: In 60 SD rats, experimental peritonitis was induced using the cecal ligation and puncture model (CLP). Animals were randomly assigned to 5 groups; ringer lactate solution (RL group), polysaccharides from PG and PL (PG and PL group), hyaluronic acid (HA group), and carboxymethylcellulose (CMC group). Intraperitoneal adhesions and abscesses were noted at 7 day after CLP. RT-PCR assay for urokinase-type plasminogen activator (uPA), its cellular receptor (uPAR), tissue-type plasminogen activator (tPA), plasminogen activator inhibitor type 1 (PAI-1), and tumor necrosis factor (TNF)- alpha was performed to assess the cecal tissue.

Results: Adhesion formation was significantly reduced in PG, PL, CMC, and HA groups (P < 0.001) compared with that in RL group. The incidence of abscesses was also significantly reduced in PG and PL groups (P < 0.05) compared with that in the RL group. The level of uPA, uPAR, tPA, and TNF-alpha was highly expressed in PG and PL group, as compared with the RL group.

Conclusions: We concluded that PG and PL had significant adhesion- and abscess-reducing effects and may act by modulating fibrinolytic capacity of uPA and/or tPA produced from macrophages in a rat peritonitis model.

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Figures

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FIGURE 1. Total adhesion score of intraperitoneal adhesion in each group (n = 12). *P < 0.001 versus RL group.
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FIGURE 2. Compare the extent of mesenteric inflammatory reaction among A, RL; B, PG; C, PL; and D, HA (× 40). Note markedly reduced inflammatory reaction in PG-treated groups (n = 6).
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FIGURE 3. The grade of inflammatory reaction in each group (n = 12). *P < 0.05 compared with RL group.
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