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. 2005 Mar;71(3):1254-8.
doi: 10.1128/AEM.71.3.1254-1258.2005.

Degradation of 1,4-dioxane and cyclic ethers by an isolated fungus

Kunichika Nakamiya  1 , Syunji Hashimoto, Hiroyasu Ito, John S Edmonds, Masatoshi Morita
Affiliations

Degradation of 1,4-dioxane and cyclic ethers by an isolated fungus

Kunichika Nakamiya et al. Appl Environ Microbiol. 2005 Mar.

Abstract

By using 1,4-dioxane as the sole source of carbon, a 1,4-dioxane-degrading microorganism was isolated from soil. The fungus, termed strain A, was able to utilize 1,4-dioxane and many kinds of cyclic ethers as the sole source of carbon and was identified as Cordyceps sinensis from its 18S rRNA gene sequence. Ethylene glycol was identified as a degradation product of 1,4-dioxane by the use of deuterated 1,4-dioxane-d8 and gas chromatography-mass spectrometry analysis. A degradation pathway involving ethylene glycol, glycolic acid, and oxalic acid was proposed, followed by incorporation of the glycolic acid and/or oxalic acid via glyoxylic acid into the tricarboxylic acid cycle.

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Figures

FIG. 1.
FIG. 1.
Light micrograph of strain A.
FIG. 2.
FIG. 2.
Time course of growth of a strain A culture with 1,4-dioxane in the medium.
FIG. 3.
FIG. 3.
Chromatograph of derivatized diols from 1,4-dioxane-d8. The solid lines indicate equal height.
FIG. 4.
FIG. 4.
Proposed degradation pathway of 1,4-dioxane. Glycolaldehyde, glyoxalate, and the TCA cycle are shown as references for the proposed pathway. M.W., molecular weight.
FIG. 5.
FIG. 5.
Effect of ethylene glycol or organic acids on utilization of 1,4-dioxane. Open symbols, cultivation on 0.034 M 1,4-dioxane with 0.034 M ethylene glycol or organic acids. Closed symbols, cultivation on 0.034 M 1,4-dioxane with 0.01 M ethylene glycol or organic acids. Circles, 1,4-dioxane; triangles, ethylene glycol or organic acids; squares, protein.
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References

    1. Bernhardt, D., and H. Diekmann. 1991. Degradation of dioxane tetrahydrofuran and other cyclic ethers by an environmental Rhodococcus strain. Appl. Microbiol. Biotechnol. 36:120-123. - PubMed
    1. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254. - PubMed
    1. Burback, B. L., and J. J. Perry. 1993. Biodegradation and biotransformation of groundwater pollutant mixtures by Mycobacterium vaccae. Appl. Environ. Microbiol. 59:1025-1029. - PMC - PubMed
    1. Clay, K. L., and R. C. Murphy. 1977. On the metabolic acidosis of ethylene glycol intoxication. Toxicol. Appl. Pharmacol. 39:39-49. - PubMed
    1. Derosa, C. T., S. Wilbur, J. Holler, P. Richter, and Y. W. Stevens. 1996. Health evaluation of 1,4-dioxane. Toxicol. Ind. Health. 12:1-43. - PubMed

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