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. 2005 Mar;43(3):1420-5.
doi: 10.1128/JCM.43.3.1420-1425.2005.

Genetic variability among serotype G4 Italian human rotaviruses

S Arista  1 , G M Giammanco, S De Grazia, C Colomba, V Martella
Affiliations

Genetic variability among serotype G4 Italian human rotaviruses

S Arista et al. J Clin Microbiol. 2005 Mar.

Abstract

A total of 254 serotype GH rotavirus strains were detected in Palermo, Italy, from 1985 to 2003. Out of 38 serotype G4 strains selected for genetic analysis, 14 were recognized by genotyping as type G9. Strains confirmed to belong to the G4 type showed temporal patterns of genetic evolution in their VP7 and VP4 gene sequences, and the latest Italian G4 strains were distantly related to the reference vaccinal ST3 strain.

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Figures

FIG. 1.
FIG. 1.
Phylogenetic analysis of partial VP7 nucleotide sequences (nt 63 to 880) of serotype G4 strains. The phylogenetic tree was constructed using the neighbor-joining method and Kimura two-parameter model. Percentage bootstrap values above 60% are given at branch nodes. Nucleotide identity (id) within lineages is indicated in parentheses to the right of the sublineage. The number of substitutions per site is indicated by the scale bar.
FIG. 2.
FIG. 2.
Comparison of amino acid sequences of VP7 VRs 3, 4, and 6 to 9. Amino acids different from those in the ST3 reference vaccinal strain are shown. Conserved amino acids are indicated by periods. The codons discriminating between subtype A (ST3-like) and B (VA75-like) (21) are indicated (▾).
FIG. 3.
FIG. 3.
Phylogenetic analysis of partial VP4 nucleotide sequences (nt 29 to 449) of type P[8] strains. The phylogenetic tree was constructed using the neighbor-joining method and Kimura two-parameter model. Type P [9] PA28 sequence was used as an outgroup. Percentage bootstrap values above 60% are given at branch nodes. Nucleotide identity (id) within lineages is indicated in parentheses to the right of the lineage. The number of substitutions per site is indicated by the scale bar.
FIG. 4.
FIG. 4.
Comparison of conserved amino acids between residues 10 and 143 of VP4. Conserved amino acids are indicated by periods. Amino acids different from those in the Wa reference vaccinal strain are shown. Amino acids related to P[8] lineage specificity according to Maunula et al. (27) and Cunliffe et al. (9) are indicated (▾).
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References

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