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. 2005 Feb;11(2):225-31.
doi: 10.3201/eid1102.041028.

Novel flavivirus or new lineage of West Nile virus, central Europe

Tamás Bakonyi  1 , Zdenek Hubálek, Ivo Rudolf, Norbert Nowotny
Affiliations

Novel flavivirus or new lineage of West Nile virus, central Europe

Tamás Bakonyi et al. Emerg Infect Dis. 2005 Feb.

Abstract

A flavivirus (strain 97-103) was isolated from Culex pipens mosquitoes in 1997 following floods in South Moravia, Czech Republic. The strain exhibited close antigenic relationship to West Nile virus (WNV) prototype strain Eg-101 in a cross-neutralization test. In this study, mouse pathogenicity characteristics and the complete nucleotide and putative amino acid sequences of isolate 97-103, named Rabensburg virus (RabV) after a nearby Austrian city, were determined. RabV shares only 75%-77% nucleotide identity and 89%-90% amino acid identity with representative strains of WNV lineages 1 and 2. Another RabV strain (99-222) was isolated in the same location 2 years later; it showed >99% nucleotide identity to strain 97-103. Phylogenetic analyses of RabV, WNV strains, and other members of the Japanese encephalitis virus (JEV) complex clearly demonstrated that RabV is either a new (third) lineage of WNV or a novel flavivirus of the JEV group.

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Figures

Figure 1
Figure 1
Phylogenetic tree illustrating the genetic relationship between selected West Nile virus strains based on their complete genome sequences. Bar on the left demonstrates the genetic distance. (Abbreviations and accession numbers are listed in Table 2.)
Figure 2
Figure 2
Phylogenetic tree illustrating the genetic relationship between representatives of the Japanese encephalitis virus complex and selected West Nile virus strains based on partial genome sequences of the NS5 protein gene. Bar on the left demonstrates the genetic distance. (Abbreviations and accession numbers are listed in Table 2.)
See this image and copyright information in PMC

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