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. 2005 Feb;11(2):298-301.
doi: 10.3201/eid1102.041016.

In vitro host-cell susceptibility to Usutu virus

Tamás Bakonyi  1 , Helga Lussy, Herbert Weissenböck, Akos Hornyák, Norbert Nowotny
Affiliations

In vitro host-cell susceptibility to Usutu virus

Tamás Bakonyi et al. Emerg Infect Dis. 2005 Feb.

Abstract

We investigated the susceptibility to Usutu virus (Flavivirus) of 13 permanent cell lines, 3 primary cell cultures, and chicken embryos. Vero, PK-15, and goose embryo fibroblast cells developed cytopathic effects; however, viral multiplication was detected in all mammalian cell types by immunohistochemical tests. Chicken embryo fibroblast cells and chicken embryos were resistant.

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Figures

Figure 1
Figure 1
Cytopathic effect (CPE) of Vero cells caused by Usutu virus infection, 4 days postinfection (hematoxylin-eosin staining). A) Uninfected control, B) Usutu virus infected. bar = 100 µm.
Figure 2
Figure 2
Demonstration of Usutu virus antigen 3 days postinfection. Immunohistochemical (IHC) tests were performed by using a polyclonal antibody to West Nile virus, which cross-reacts with Usutu virus. A) Vero control; B) Vero infected; C) CR [define] (feline) control; D) CR infected; E) goose embryo fibroblast (GEF) [define] control; F) GEF infected; A,B) bar = 50 µm; C–F) bar = 100 µm. IHC staining.
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