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. 2005 Feb 28;11(8):1237-40.
doi: 10.3748/wjg.v11.i8.1237.

Expression of p57(kip2) and its relationship with clinicopathology, PCNA and p53 in primary hepatocellular carcinoma

Ke-Jun Nan  1 , Hui Guo, Zhi-Ping Ruan, Zhao Jing, Shaan-Xi Liu
Affiliations

Expression of p57(kip2) and its relationship with clinicopathology, PCNA and p53 in primary hepatocellular carcinoma

Ke-Jun Nan et al. World J Gastroenterol. .

Abstract

Aim: To investigate the expression of p57(kip2) and its relationship with clinicopathology, PCNA and p53 in primary hepatocellular carcinoma (HCC).

Methods: Expression of p57(kip2), PCNA and p53 in tumor tissues from 32 patients with HCC and 10 liver tissues of normal persons was detected with Elivision immunohistochemical technique.

Results: The p57(kip2) protein positive-expression rate in HCC was 56.25%, lower than that in normal tissues (100%, P<0.05). The reduced expression of p57(kip2) protein correlated significantly with moderate or low differentiation of tumor cells (P = 0.007 <0.05), high clinical stage (P = 0.041 <0.05) and poor prognosis (P = 0.036 <0.05), but did not correlate significantly with metastasis, tumor size, level of AFP and age (P>0.05). The PCNA positive-expression rate was 56.25%, which was correlated significantly with the expression of p57(kip2) (P = 0.025<0.05). The p53 positive-expression rate was 46.88%, which was not correlated significantly with the expression of p57(kip2) (P>0.05).

Conclusion: There is a marked loss or absence of p57(kip2) expression and high expression of PCNA in HCC, which are involved in carcinogenesis and development of HCC. The p57(kip2) and p53 may induce apoptosis via different mechanisms.

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Figures

Figure 1
Figure 1
Positive p57kip2 expression in normal hepatocytes and well-differentiated carcinomas. A: Positive p57kip2 expression in normal hepatocytes (20×); B: Positive p57kip2 expression in well-differentiated carcinomas (40×).
Figure 2
Figure 2
Negative p57kip2 expression in moderately or poorly differentiated carcinomas and positive expression of p57kip2 in adjacent liver tissues (20×).
Figure 3
Figure 3
Highly positive expression of PCNA in tumor tissue of HCC and low expression of PCNA in adjacent liver tissues (40×).
Figure 4
Figure 4
Positive expression of p53 in HCC tissue (40×).
See this image and copyright information in PMC

References

    1. Matsuoka S, Edwards MC, Bai C, Parker S, Zhang P, Baldini A, Harper JW, Elledge SJ. p57KIP2, a structurally distinct member of the p21CIP1 Cdk inhibitor family, is a candidate tumor suppressor gene. Genes Dev. 1995;9:650–662. - PubMed
    1. Yue H, Na YL, Feng XL, Ma SR, Song FL, Yang B. Expression of p57kip2, Rb protein and PCNA and their relationships with clinicopathology in human pancreatic cancer. World J Gastroenterol. 2003;9:377–380. - PMC - PubMed
    1. Zhu MH, Ni CR, Zhu Z, Li FM, Zhang SM. Determination of expression of eight p53-related genes in hepatocellular carcinoma with tissue microarrays. AiZheng. 2003;22:680–685. - PubMed
    1. Lee MH, Yang HY. Negative regulators of cyclin-dependent kinases and their roles in cancers. Cell Mol Life Sci. 2001;58:1907–1922. - PMC - PubMed
    1. Li Y, Millikan RC, Newman B, Conway K, Tse CK, Liu ET. P57 (KIP2) polymorphisms and breast cancer risk. Hum Genet. 1999;104:83–88. - PubMed

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