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. 2005 Apr;6(4):227-31.
doi: 10.1631/jzus.2005.B0227.

An integrated approach utilizing proteomics and bioinformatics to detect ovarian cancer

Jie-kai Yu  1 , Shu Zheng, Yong Tang, Li Li
Affiliations

An integrated approach utilizing proteomics and bioinformatics to detect ovarian cancer

Jie-kai Yu et al. J Zhejiang Univ Sci B. 2005 Apr.

Abstract

Objective: To find new potential biomarkers and establish the patterns for the detection of ovarian cancer.

Methods: Sixty one serum samples including 32 ovarian cancer patients and 29 healthy people were detected by surface-enhanced laser desorption/ionization mass spectrometry (SELDI-MS). The protein fingerprint data were analyzed by bioinformatics tools. Ten folds cross-validation support vector machine (SVM) was used to establish the diagnostic pattern.

Results: Five potential biomarkers were found (2085 Da, 5881 Da, 7564 Da, 9422 Da, 6044 Da), combined with which the diagnostic pattern separated the ovarian cancer from the healthy samples with a sensitivity of 96.7%, a specificity of 96.7% and a positive predictive value of 96.7%.

Conclusions: The combination of SELDI with bioinformatics tools could find new biomarkers and establish patterns with high sensitivity and specificity for the detection of ovarian cancer.

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Figures

Fig. 1
Fig. 1
The accuracies of SVMs combined with different peaks
Fig. 2
Fig. 2
The spectra and gel maps of potential biomarkers. (a) The spectrum and the gel map of 2085 Da; (b) The spectrum and the gel map of 5881 Da; (c) The spectrum and the gel map of 7564 Da; (d) The spectrum and the gel map of 9422 Da; (e) The spectrum and the gel map of 6044 Da
Fig. 2
Fig. 2
The spectra and gel maps of potential biomarkers. (a) The spectrum and the gel map of 2085 Da; (b) The spectrum and the gel map of 5881 Da; (c) The spectrum and the gel map of 7564 Da; (d) The spectrum and the gel map of 9422 Da; (e) The spectrum and the gel map of 6044 Da
Fig. 2
Fig. 2
The spectra and gel maps of potential biomarkers. (a) The spectrum and the gel map of 2085 Da; (b) The spectrum and the gel map of 5881 Da; (c) The spectrum and the gel map of 7564 Da; (d) The spectrum and the gel map of 9422 Da; (e) The spectrum and the gel map of 6044 Da
Fig. 2
Fig. 2
The spectra and gel maps of potential biomarkers. (a) The spectrum and the gel map of 2085 Da; (b) The spectrum and the gel map of 5881 Da; (c) The spectrum and the gel map of 7564 Da; (d) The spectrum and the gel map of 9422 Da; (e) The spectrum and the gel map of 6044 Da
Fig. 2
Fig. 2
The spectra and gel maps of potential biomarkers. (a) The spectrum and the gel map of 2085 Da; (b) The spectrum and the gel map of 5881 Da; (c) The spectrum and the gel map of 7564 Da; (d) The spectrum and the gel map of 9422 Da; (e) The spectrum and the gel map of 6044 Da
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