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. 2004 Nov;30(3):245-51.
doi: 10.1007/s10858-005-1679-2.

(13)C-(13)C NOESY: a constructive use of (13)C-(13)C spin-diffusion

Ivano Bertini  1 , Isabella C Felli, Rainer Kümmerle, Claudio Luchinat, Roberta Pierattelli
Affiliations

(13)C-(13)C NOESY: a constructive use of (13)C-(13)C spin-diffusion

Ivano Bertini et al. J Biomol NMR. 2004 Nov.

Abstract

(13)C-(13)C NOESY experiments were performed under long mixing time conditions on reduced human superoxide dismutase (32 kDa, (15)N, (13C) and 70% (2)H labeled). (13)C-(13)C couplings were successfully eliminated through post-processing of in-phase-anti-phase (IPAP) data. It appears that at mixing time tau(m) of 3.0 s the spin diffusion mechanism allows the detection of 96% of the two-bond correlations involving C' and C(beta). The interpretation was confirmed by simulations. This approach broadens the range of applicability of (13)C-(13)C NOESY spectroscopy.

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