Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Sep 13;5(4):e53.
doi: 10.1208/pt050453.

Particle size and temperature effect on the physical stability of PLGA nanospheres and microspheres containing Bodipy

Sinjan De  1 , Dennis H Robinson
Affiliations

Particle size and temperature effect on the physical stability of PLGA nanospheres and microspheres containing Bodipy

Sinjan De et al. AAPS PharmSciTech. .

Abstract

The purpose of this study was to investigate the effect of particle size, storage temperature, and duration of storage on the physical stability and morphology of polylactic-co-glycolic acid (PLGA) nanospheres and microspheres. PLGA nanospheres and microspheres containing the fluorescent dye, Bodipy, were prepared in varying sizes by controlling the method and degree of agitation during the emulsification phase of preparation. Mean diameters of the particles were measured by dynamic light scattering. To evaluate the effect of storage temperature and duration of storage on the extent of aggregation, nanospheres and microspheres were stored at 4 degrees C, 25 degrees C, 37 degrees C, and 50 degrees C for 6 days and then monitored using both confocal and scanning electron microscopy. The mean +/- SD diameters of PLGA particles containing Bodipy were: 266.9 +/- 2.8, 351.6 +/- 1.8, 988.8 +/- 14.1, and 1865.9 +/- 67.0 nm. The extent of aggregation of the particulate delivery system decreased as the mean diameter increased, and increased as the storage temperature increased. The maximum extent of aggregation was observed with the smallest (266 nm) nanospheres. Microspheres did not aggregate. The aggregation of nanospheres was significantly reduced by introducing an additional evaporation step during preparation, suggesting that migration of residual dichloromethane from within the nanospheres may have dissolved the PLGA on the surface. The extent of aggregation of nanospheres increased as the temperature was increased from 4 degrees C to 50 degrees C, and decreased as particle size increased. To avoid aggregation, PLGA nanospheres should be stored at 4 degrees C.

PubMed Disclaimer

References

    1. De S, Robinson D. Polymer relationships during preparation of chitosan-alginate and poly-1-lysine-alginate nanospheres. J Control Release. 2003;89:101–112. doi: 10.1016/S0168-3659(03)00098-1. - DOI - PubMed
    1. Ricci M, Blasi P, Giovagnoli S, Perioli L, Vescovi C, Rossi C. Leucinostatin—A loaded nanospheres: characterization and in vivo toxicity and efficacy evaluation. Int J Pharm. 2004;275:61–72. doi: 10.1016/j.ijpharm.2004.01.030. - DOI - PubMed
    1. Panyam J, Zhou WZ, Prabha S, Sahoo SK, Labhasetwar V. Rapid endo-lysosomal escape of poly(DL-lactide-co-glycolide) nanoparticles: implications for drug and gene delivery. FASEB J. 2002;16:1217–1226. doi: 10.1096/fj.02-0088com. - DOI - PubMed
    1. Muller M, Voros J, Csucs G, et al. Surface modification of PLGA microspheres. J Biomed Mater Res. 2003;66A:55–61. doi: 10.1002/jbm.a.10502. - DOI - PubMed
    1. Feng S, Huang G. Effects of emulsifiers on the controlled release of paclitaxel (Taxol) from nanospheres of biodegradable polymers. J Control Release. 2001;71:53–69. doi: 10.1016/S0168-3659(00)00364-3. - DOI - PubMed

Publication types

LinkOut - more resources