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. 2005 Jan;29(1):15-9.
doi: 10.1016/j.cellbi.2004.11.003. Epub 2005 Jan 26.

Production of transgenetic sugarbeet (Beta vulgaris L.) plants resistant to phosphinothricin

E M Kishchenko  1 , I K Komarnitskii, N V Kuchuk
Affiliations

Production of transgenetic sugarbeet (Beta vulgaris L.) plants resistant to phosphinothricin

E M Kishchenko et al. Cell Biol Int. 2005 Jan.

Abstract

A method of Agrobacterium-mediated genetic transformation of sugarbeet (Beta vulgaris L.) with vacuum infiltration has been developed. Aseptic 3-weeks old etiolated seedlings of two diploid O-type sugarbeet lines (KS3 and KS7) have been used for genetic transformation. Transgenic sugarbeet plants carrying the reporter beta-glucuronidase gene have been selected for their resistance to glufosinate ammonium herbicide. Integration of transgenes into sugarbeet genome was confirmed with GUS assay and PCR using primers for bar and gusA genes.

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