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. 2005 Mar 14;11(10):1457-62.
doi: 10.3748/wjg.v11.i10.1457.

Are polymorphisms of N-acetyltransferase genes susceptible to primary liver cancer in Luoyang, China?

Xiu-Feng Zhang  1 , Jian-Chao Bian, Xiao-Yan Zhang, Zhu-Mei Zhang, Feng Jiang, Qi-Min Wang, Qi-Jun Wang, Yan-Yan Cao, Bo-Ming Tang
Affiliations

Are polymorphisms of N-acetyltransferase genes susceptible to primary liver cancer in Luoyang, China?

Xiu-Feng Zhang et al. World J Gastroenterol. .

Abstract

Aim: To identify whether the polymorphisms of the N-acetyltransferase (NAT) genes are susceptible to primary liver cancer (PLC) in Luoyang, a PLC low-incidence area of China.

Methods: The NAT1 and NAT2 genotypes of 96 PLC cases and 173 controls were determined by PCR-RFLP. Both interaction between NAT1 or NAT2 and environmental risk factors were analyzed based on case control study.

Results: Compared to the control group, the frequencies of alleles NAT1*3, NAT1*4, NAT1*10, NAT1*14B and alleles NAT2*4, NAT2*6, NAT2*7 in PLC group showed no statistically significant difference (chi(2) = 2.61 and 4.16, respectively, both P>0.05). The frequencies of NAT1 genotypes NAT1*3/*3, NAT1*3/*4, NAT1*3/*10, NAT1*3/*14B, NAT1*4/*4, NAT1*4/*10, NAT1*4/*14B, NAT1*10/*10, NAT1*10/*14B, and NAT2 genotypes NAT2*4/*4, NAT2*4/*6, NAT2*4/*7, NAT2*6/*6, NAT2*6/*7 and NAT2*7/*7 also had no statistically significant difference between the two groups (chi(2) = 11.86 and 2.94 respectively both, P>0.05). Neither the frequencies of rapid and slow NAT1 acetylators nor the frequencies of rapid and slow NAT2 acetylators were significantly different between the two groups (chi(2) = 0.598 and 0.44, respectively, both P>0.05). The interaction between NAT1*10 and occupational exposures was found significant with an odds ratio of 3.40 (chi(2) = 8.42, P = 0.004, OR 95%CI:1.03-11.22). But no interaction was found between NAT2 and any environmental risk factors.

Conclusion: The polymorphisms of NAT1 and NAT2 are not susceptible to PLC in Luoyang. Allele NAT1*10 interacts with occupational exposures.

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Figures

Figure 1
Figure 1
RFLP product of NAT1 C1095A site digested by Alw26I. Lanes 1, 3, 4, 6, 8, and 9: wild homozygotes; lanes 2, 7: heterozygotes; lanes 5, 10: mutant homozygotes; M: 100 bp DNA Ladder I; the fragments from up to down are 600, 500, 400, 300, 200 and 100 bp, respectively.
Figure 2
Figure 2
RFLP product of NAT1 G560A site digested by HinfI. Lanes 1, 6, 7: heterozygotes; lane 2: wild homozygote; lanes 3, 4, 5: mutant homozygotes; M: pGEM-7Zf(+) DNA/HaeIII Markers; the fragments from up to down are 657, 458, 434, 328, 289, 267, 174, 142, 102 and 80 bp.
Figure 3
Figure 3
RFLP product of NAT1 T1088A site digested by Ase I. Lanes 1, 5: wild homozygotes; lanes 2, 3, 6, 7: heterozygotes; lanes 4, 8: mutant homozygotes; M: 100 bp DNA Ladder I; the fragments from up to down are 600, 500, 400, 300, 200 and 100 bp respectively.
Figure 4
Figure 4
RFLP product of NAT2 G560A site digested by Taq I. Lanes 1, 4: NAT2*4/*6; lane 3: NAT2*6/*6; lanes 2, 5: NAT2*4/*4; M: 50 bp DNA Ladder; the fragments from up to down are 500, 300, 200, 150, 100 and 50 bp.
Figure 5
Figure 5
RFLP product of NAT2 G857A site digested by BamHI. Lanes 1, 4, 6: NAT2*4/*7; lane 5: NAT2*7/*7; lanes 2, 3, 7: NAT2*4/*4; M: DNA Ladder Mix; the fragments from up to down are 3000, 2000, 1500, 1200, 1031, 900, 800, 700, 600 and 500 bp.
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