Spatial navigation in the Morris water maze: working and long lasting reference memories

Abstract

Spatial navigation development in the Morris water maze (MWM) paradigm was studied in 70-day-old male Long-Evans hooded rats. During 5 consecutive days, rats' training consisted of a daily block of 10 trials. Escape latency was measured in each trial. Probe testing was performed every day immediately before and after the daily block of trials. In addition, a final probe was performed on Day 6. During the first 3 days of training, the escape latency became progressively shorter, showing an asymptotic trend on Days 4 and 5. Probe trials administered at the end of the first acquisition sessions showed clear preference for the target quadrant but this information was not recalled at probe trials given 24h later. The memory trace retrieved after 24-h delay was formed only after 30 trials received over three sessions. The probe trial given at the end of an acquisition session tests the efficiency of the working memory whereas the 24h delayed probe trial reflects better-consolidated spatial information corresponding to long lasting reference memory. It can be noted that the progressive shortening of escape latencies does not express closely the evolution of the rat's long lasting (consolidated) reference memory. This memory can be satisfactorily measured only by probe testing performed at an adequate delay after training. These considerations may be of some interest when interpreting the rat's performance in the MWM.