Review
doi: 10.1016/j.patbio.2004.12.001.
Ex vivo amplification of T cells from human cord blood
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- PMID: 15781374
- DOI: 10.1016/j.patbio.2004.12.001
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Review
Ex vivo amplification of T cells from human cord blood
Pathol Biol (Paris).
2005 Apr.
Abstract
The current status of immunotherapy for cancer is here summarized with particular attention to the new methodologies developed for ex vivo expansion of T cells from neonatal cord blood. Umbilical cord blood (UCB) mononuclear cells (MNC) generate CD45RA naive T lymphocytes when cultured under serum-deprived conditions with appropriate combinations of growth factors. These ex vivo generated T cells resemble precursors for the lymphoid lineage present in adult bone marrow in terms of active transcription of RAG-2 and pTalpha.
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