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. 2004 Oct;1(1):27-9.
doi: 10.1038/nmeth708.

A rapid method for determining protein kinase phosphorylation specificity

Jessica E Hutti  1 , Emily T Jarrell, James D Chang, Derek W Abbott, Peter Storz, Alex Toker, Lewis C Cantley, Benjamin E Turk
Affiliations

A rapid method for determining protein kinase phosphorylation specificity

Jessica E Hutti et al. Nat Methods. 2004 Oct.

Abstract

Selection of target substrates by protein kinases is strongly influenced by the amino acid sequence surrounding the phosphoacceptor site. Identification of the preferred peptide phosphorylation motif for a given kinase permits the production of efficient peptide substrates and greatly simplifies the mapping of phosphorylation sites in protein substrates. Here we describe a combinatorial peptide library method that allows rapid generation of phosphorylation motifs for serine/threonine kinases.

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Comment in

  • Novel at the library.
    Yaffe MB. Yaffe MB. Nat Methods. 2004 Oct;1(1):13-4. doi: 10.1038/nmeth1004-13. Nat Methods. 2004. PMID: 15782146 No abstract available.

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