Modulation of hair cell voltage responses to tones by low-frequency biasing of the basilar membrane in the guinea pig cochlea

Abstract

Inner (IHC) and outer (OHC) hair cell receptor potentials were recorded during stimulation with combinations of high-frequency (HF) tones and a 100 Hz tone burst of constant level (80 dB SPL). For frequencies at and below characteristic frequency (CF), OHC AC receptor potentials were suppressed by the 100 Hz tone at levels of the HF tone below about 70 dB SPL (the initial steep part of the AC/level function) and at levels that were frequency specific for frequencies above CF. Suppression was associated with a phase lead for frequencies at and close to the CF. For frequencies above CF, the OHC AC response was either suppressed or augmented at levels of the HF tone both below and above 70 dB SPL, depending on the frequency. The action of the 100 Hz tone on the AC response/level functions was to change nonmonotonic functions into monotonic functions or vice versa. IHC DC receptor potentials were suppressed maximally at the CF and at levels and frequencies where suppression of the OHC AC response and the appearance of the IHC DC response overlapped. For levels of the HF tone above 70 dB SPL, the amplitude of the responses of both IHCs and OHCs to the 100 Hz tone are suppressed and become more symmetrical through selective attenuation of the depolarizing phase of the IHC response and the hyperpolarizing phase of the OHC response. In IHCs from insensitive preparations, the response to the 100 Hz tone is augmented in the presence of the HF tone, which may indicate a shift in the operating point of transduction. At frequencies about one-half an octave below the CF, the phase of the 100 Hz voltage response of OHCs but not IHCs is inverted for levels of the HF tone above about 90 dB SPL. It is proposed that amplitude and phase changes in the response to the HF tone due to the presence of the 100 Hz tone are the result of changes in OHC feedback processes and in the mode of movement of the interface between OHC stereocilia and tectorial membrane. The interaction between the responses to HF and 100 Hz tones indicates that feedback contributes significantly to the voltage responses of OHCs throughout the frequency response range.