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. 2005 Mar 25:2:12.
doi: 10.1186/1742-4682-2-12.

Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics approach

D F Harney  1 , R K Butler, R J Edwards
Affiliations

Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics approach

D F Harney et al. Theor Biol Med Model. .

Abstract

Background: Previously it has been shown that insulin-mediated tyrosine phosphorylation of myosin heavy chain is concomitant with enhanced association of C-terminal SRC kinase during skeletal muscle differentiation. We sought to identify putative site(s) for this phosphorylation event.

Results: A combined bioinformatics approach of motif prediction and evolutionary and structural analyses identified tyrosines163 and 1856 of the skeletal muscle heavy chain as the leading candidate for the sites of insulin-mediated tyrosine phosphorylation.

Conclusion: Our work is suggestive that tyrosine phosphorylation of myosin heavy chain, whether in skeletal muscle or in platelets, is a significant event that may initiate cytoskeletal reorganization of muscle cells and platelets. Our studies provide a good starting point for further functional analysis of MHC phosphor-signalling events within different cells.

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Figures

Figure 1
Figure 1
Neighbour-joining phylogeny of MHC homologues, with bootstrap support. PDB structure 2MYS is marked with a black diamond.
See this image and copyright information in PMC

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