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. 2005 Apr;58(4):429-31.
doi: 10.1136/jcp.2004.020131.

Immunodetection of SV40 large T antigen in human central nervous system tumours

J Sabatier  1 , E Uro-Coste, A Benouaich, S Boetto, M Gigaud, M Tremoulet, M-B Delisle, F Galateau-Sallé, P Brousset
Affiliations

Immunodetection of SV40 large T antigen in human central nervous system tumours

J Sabatier et al. J Clin Pathol. 2005 Apr.

Abstract

Background/aims: DNA sequences from Simian virus 40 (SV40) have been previously isolated from various human tumours of the central nervous system (CNS). This study aimed to investigate a series of tumours of the CNS for the expression of the SV40 large T antigen (Tag), which is an oncogenic protein of the virus.

Methods: A French series of 82 CNS tumours was investigated for Tag expression using a monoclonal antibody and immunohistochemistry. A Tag positive hepatocellular carcinoma cell line from transgenic mice and a kidney biopsy from a patient infected by SV40 were used as positive controls.

Results: None of the tumours (20 ependymomas, 20 glioblastomas, 12 oligodendrogliomas, three plexus choroid adenomas, two plexus choroid carcinomas, 15 meningiomas, and 10 medulloblastomas) contained SV40 Tag positive cells.

Conclusions: The lack of SV40 Tag in 82 CNS tumours of various types is at variance with previous studies from different countries, and suggests that the virus may not be an important factor in CNS tumorigenesis, at least in French cases.

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Figures

Figure 1
Figure 1
(A) Positive nuclear immunostaining of the formalin fixed and paraffin wax embedded hepatocellular carcinoma cell line with a monoclonal anti-Simian virus 40 (SV40) large T antigen (Tag) antibody (Ab-2). Horseradish peroxidase; original magnification, ×1000. (B) Positive immunostaining for Tag (Ab-2 antibody) seen in a paraffin wax embedded kidney biopsy section from a transplant patient with active SV40 infection. Nuclear staining of dystrophic tubular cells. Horseradish peroxidase; original magnification, ×1000.
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