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. 2005 May 5;819(1):59-66.
doi: 10.1016/j.jchromb.2005.01.022.

Peptide profiling in epithelial tumor plasma by the emerging proteomic techniques

Emilia Caputo  1 , Maria Luisa Lombardi, Vincenza Luongo, Ramy Moharram, Pete Tornatore, Giuseppe Pirozzi, John Guardiola, Brian M Martin
Affiliations

Peptide profiling in epithelial tumor plasma by the emerging proteomic techniques

Emilia Caputo et al. J Chromatogr B Analyt Technol Biomed Life Sci. .

Abstract

The plasma peptide component (PPC) from ten melanoma (Mel), breast cancer (BC) and healthy individuals was examined by a combination of RP-HPLC, surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) and tandem mass spectrometry. A three peak pattern (2023, 2039, 2053.5 m/z) was primarily observed in melanoma. Two peaks (2236.1 and of 2356.3 m/z) were found only in BC samples. Fibrinogen alpha and inter-alpha-trypsin inhibitor heavy chain H4 fragments were absent in both tumor samples.

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