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. 2005 Apr 7;11(13):2000-3.
doi: 10.3748/wjg.v11.i13.2000.

Analysis of metastasis suppressing function of E-cadherin in gastric cancer cells by RNAi

Zhi-Hong Zheng  1 , Xiu-Ju Sun, Hai-Tao Zhou, Chao Shang, Hong Ji, Kai-Lai Sun
Affiliations

Analysis of metastasis suppressing function of E-cadherin in gastric cancer cells by RNAi

Zhi-Hong Zheng et al. World J Gastroenterol. .

Abstract

Aim: To study the effect of inhibited E-cadherin expression on invasion of cancer cells.

Methods: We designed the nucleotide sequence of siRNA corresponding to 5' non-coding and coding sequence of E-cadherin. 21-nucleotide dssiRNA was synthesized by in vitro transcription with Ambion Silencer TM siRNA Construction Kit. siRNA was transfected into gastric cancer MKN45 using TransMessenger transfection Kit. RT-PCR and immunofluorescent assay were used to investigate the inhibition of the expression of mutated E-cadherin. Invasive ability of cancer cells was determined by Transwell assay.

Results: The synthesis of E-cadherin mRNA rather than protein expression was suppressed dramatically 7 d after interference. Decreased protein expression was observed on d 10 after interference. On d 11, invasion ability was enhanced significantly.

Conclusion: siRNA targeted at non-coding and coding sequence of E-cadherin showed significant inhibition on mRNA and protein expression. Inhibited E-cadherin expression results in increased invasion ability of cancer cells.

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Figures

Figure 1
Figure 1
A: The significant suppression of mRNA expression in MKN45 before and after RNAi (lanes 1 and 2) compared with the control cells (lane 3); B: β-actin control; 1: IIsiRNA, 2: IsiRNA 3: control, 4: DL2000.
Figure 2
Figure 2
Downregulated E-cadherin expression (C): On the 12th d of interference; B: On the 10th d of interference) in MKN45 cells undergoing RNAi compared with that in control group (A).
See this image and copyright information in PMC

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