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Comparative Study
. 2005 Mar;37(1):52-7.

Thrombelastograph (TEG) analysis of platelet gel formed with different thrombin concentrations

William Cory Ellis  1 , Linsay K Cassidy, Angela S Finney, Allison J Spiwak, Jeffrey B Riley
Affiliations
Comparative Study

Thrombelastograph (TEG) analysis of platelet gel formed with different thrombin concentrations

William Cory Ellis et al. J Extra Corpor Technol. 2005 Mar.

Abstract

Autologous blood transfusion is the safest and most successful way to decrease transfusion-related risks such as postoperative infections, allo-immunization, and short- and long-term immunosuppression. In addition, these fibrin sealants are known to provide coagulation support at the surgical site and act as an adjunct to the control of postoperative bleeding. The physical formation of autologous platelet fibrin gel clot is dependent on both the common pathway of the coagulation cascade and platelet activation. Platelet gel can help provide control of intraoperative and postoperative bleeding. The Thrombelastograph Hemostasis Analyzer (TEG) measures the viscoelastic properties of a clot as it forms. Based on the information that the TEG provides, it promises to be a good choice for point of care measurement of the integrity of thrombus formed by platelet gels. Bovine blood from a single donor was sequestered into platelet-rich plasma and was made into platelet gel using calcium and three different concentrations of thrombin. The platelet gel samples were then analyzed with the TEG analyzer. The results for MA, tMA, CI, and angle were recorded and statistical analysis was performed to accept or reject the null hypothesis, which is: There is no difference between TEG parameters when analyzing platelet gels formed with calcium chloride, platelet-rich plasma and three different concentrations of thrombin A one-way analysis of variance test was performed between thrombin concentrations for MA (p = 0.19), tMA (p = 0.443), CI (p = 0.257), and angle (p = 0.323). The results showed that thrombin concentration did not affect the MA, tMA, CI, or angle as measured by the TEG analyzer. The null hypothesis was accepted. Based on a one-way analysis of variance test for MA, tMA, CI, and angle there was no significant statistical difference for the TEG samples in this experiment as reported with a 95% confidence interval.

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Conflict of interest statement

The senior author has stated that authors have reported no material, financial or other relationship with any healthcare-related business or other entity whose products or services are discussed in this paper.

Figures

Figure 1.
Figure 1.
Platelet gel clotting cascade.
Figure 2.
Figure 2.
Circuit diagram.
Figure 3.
Figure 3.
TEG® tracings: low-thrombin concentration.
Figure 4.
Figure 4.
TEG® tracings: medium thrombin concentration.
Figure 5.
Figure 5.
TEG® tracings: high thrombin concentration.
Figure 6.
Figure 6.
Boxplots of MA (mm) by thrombin concentration.
Figure 7.
Figure 7.
Boxplots of tMA (sec) by thrombin concentration.
Figure 8.
Figure 8.
Boxplots of Cl by thrombin concentration.
Figure 9.
Figure 9.
Boxplots of angle (deg) by thrombin concentration (p = 0.433).
See this image and copyright information in PMC

References

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