[The construction of recombinant plasmid with prostate-specific membrane antigen promoter controlling reporter gene expression]

Abstract

Objective: To study the specificity of prostate-specific membrane antigen (PSMA) promoter in controlling gene expression.

Methods: PSMA promoter gene was amplified with PCR, and then the promoter gene was cloned into the vector pEGFP-1 to construct a recombinant plasmid, which was transfected into different cell lines such as LNcap, PC-3, MCF-7, A549. Green fluorescent protein (GFP) expression was observed.

Results: The recombinant plasmid constructed, and PSMA promoter uniquely showed modulating activity in PSMA positive cell line.

Conclusion: PSMA promoter possesses PSMA positive cell specificity, as well as prostatic tissue specificity. PSMA promoter may have the potential for use in targeted gene therapy of prostate adenocarcinoma.