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. 2005 May;140(2):289-92.
doi: 10.1111/j.1365-2249.2005.02767.x.

Lymphocyte subsets in term and significantly preterm UK infants in the first year of life analysed by single platform flow cytometry

J E Berrington  1 , D Barge, A C Fenton, A J Cant, G P Spickett
Affiliations

Lymphocyte subsets in term and significantly preterm UK infants in the first year of life analysed by single platform flow cytometry

J E Berrington et al. Clin Exp Immunol. 2005 May.

Abstract

This observational study describes the ranges observed for lymphocyte subsets for significantly preterm infants (<32 weeks) in the first year of life, measured by single platform flow cytometry and compared to identically determined subsets in term infants. After ethical approval 39 term and 28 preterm infants had lymphocyte subset analysis before and after their primary immunization series. Median values with 5th and 95th percentiles of absolute counts and percentages are presented for total lymphocytes, T cells, NK cells, B cells, cytotoxic T cells, helper T cells, dual positive T cells, activated T cells, activated T helper cells (including T regulatory cells), pan memory T cells, pan naive T cells, memory helper T cells, naive helper T cells and the T helper/suppressor ratio. The lymphocyte profile of the preterm infants differed from that of the term infants.

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References

    1. Barnett D, Granger V, Whitby L, Storie I, Reilly JT. Absolute CD4+ T-lymphocyte and CD34+ stem cell counts by single-platform flow cytometry: the way forward. Br J Haematol. 1999;106:1059–62. - PubMed
    1. de Vries E, de Bruin-Versteeg S, Comans-Bitter WM, de Groot R, Boerma GJ, Lotgering FK, et al. Correction for erythroid cell contamination in microassay for immunophenotyping of neonatal lymphocytes. Arch Dis Child: Fetal Neonatal Ed. 1999;80:F226–9. - PMC - PubMed
    1. Mansour I, Bourin P, Rouger P, Doinel C. A rapid technique for lymphocyte preparation prior to two colour immunofluorescence analysis of lymphocyte subsets using flow cytometry: comparison with density gradient separation. J Immunol Meth. 1998;127:61–70. - PubMed
    1. de Vries E, de Bruin-Versteeg S, Comans-Bitter WM, et al. Neonatal blood lymphocyte subpopulations: a different perspective when using absolute counts. Biol Neonate. 2000;77:230–5. - PubMed
    1. Comans-Bitter WM, de Groot R, van den Beemd R, et al. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatr. 1997;130:388–93. - PubMed

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