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Review
. 2005 Apr;9(2):210-6.
doi: 10.1016/j.cbpa.2005.02.002.

High-throughput screens and selections of enzyme-encoding genes

Amir Aharoni  1 , Andrew D Griffiths, Dan S Tawfik
Affiliations
Review

High-throughput screens and selections of enzyme-encoding genes

Amir Aharoni et al. Curr Opin Chem Biol. 2005 Apr.

Abstract

The availability of vast gene repertoires from both natural sources (genomic and cDNA libraries) and artificial sources (gene libraries) demands the development and application of novel technologies that enable the screening or selection of large libraries for a variety of enzymatic activities. We describe recent developments in the selection of enzyme-coding genes for directed evolution and functional genomics. We focus on HTS approaches that enable selection from large libraries (>10(6) gene variants) with relatively humble means (i.e. non-robotic systems), and on in vitro compartmentalization in particular.

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