Nucleotide and derived amino acid sequence of the subtilisin from the antarctic psychrotroph Bacillus TA39
- PMID: 1581352
- DOI: 10.1016/0167-4781(92)90108-c
Nucleotide and derived amino acid sequence of the subtilisin from the antarctic psychrotroph Bacillus TA39
Abstract
The nucleotide sequence of the subtilisin-encoding gene from the antarctic psychrotroph Bacillus TA39 was determined. The primary structure of the subtilisin precursor is composed of 420 amino acids giving rise to a mature enzyme of 309 amino acids. Asp-145, His-185 and Ser-361 are the proposed catalytic residues of the active site.
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