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Multicenter Study
. 2005 Apr;43(4):1570-4.
doi: 10.1128/JCM.43.4.1570-1574.2005.

European multicenter study of the LIAISON automated diagnostic system for determination of Toxoplasma gondii-specific immunoglobulin G (IgG) and IgM and the IgG avidity index

Eskild Petersen  1 , Maria Victoria Borobio, Edward Guy, Oliver Liesenfeld, Valeria Meroni, Anne Naessens, Emma Spranzi, Philippe Thulliez
Affiliations
Multicenter Study

European multicenter study of the LIAISON automated diagnostic system for determination of Toxoplasma gondii-specific immunoglobulin G (IgG) and IgM and the IgG avidity index

Eskild Petersen et al. J Clin Microbiol. 2005 Apr.

Abstract

The LIAISON system is a fully automated system based on chemiluminescence and antigen bound to magnetic microparticles. The system allows fast and precise measurement of Toxoplasma-specific immunoglobulin G (IgG) and IgM antibody levels and measurement of the IgG avidity index even at low levels of Toxoplasma-specific IgG antibodies in a single step without manual interference. Seven European centers participated in a multicenter evaluation of the LIAISON system. The sensitivity and specificity of the LIAISON system compared to the Sabin-Feldman dye test were 99.3 and 96.8%, respectively. In a comparison of the LIAISON Toxoplasma-specific IgM assay with an immunosorbent agglutination assay, the LIAISON assay had a sensitivity of 96.7% and a specificity of 95.4%. The LIAISON IgG assay showed agreements of 91, 100, and 100% with the AXSYM IgG (Abbott), VIDAS IgG (bioMerieux), and Platelia IgG (Bio-Rad) assays, respectively. The LIAISON IgM assay showed agreements of 95% with the AXSYM IgM and Platelia IgM assays, 96% with the ISAGA IgM assay (bioMerieux), and 97% with the VIDAS IgM assay. The coefficient of correlation between the LIAISON system and the VIDAS Toxoplasma-specific IgG avidity index was 0.81. By use of the Toxoplasma-specific IgG avidity index assay with specific IgM-positive samples, the diagnosis of infection with Toxoplasma gondii in early pregnancy has been improved significantly. The LIAISON avidity assay is a valuable assay for the exclusion of recently acquired infection with T. gondii (less than 4 months) in pregnant women, and it decreases significantly the necessity for follow-up testing.

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Figures

FIG. 1.
FIG. 1.
Development of the LIAISON IgG avidity index in 103 pregnant and treated women monitored after infection (seroconversion) (Brussels, Paris, and Pavia). The cutoff levels are indicated for the IgG avidity index and for the time after infection (see Materials and Methods).
FIG. 2.
FIG. 2.
Correlation (r = 0.81) between the T. gondii-specific IgG avidity indexes measured by the LIAISON and VIDAS systems.
See this image and copyright information in PMC

References

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