Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Apr;43(4):1612-6.
doi: 10.1128/JCM.43.4.1612-1616.2005.

Rapid detection of ofloxacin resistance in Mycobacterium tuberculosis by two low-cost colorimetric methods: resazurin and nitrate reductase assays

Anandi Martin  1 , Juan Carlos Palomino, Françoise Portaels
Affiliations

Rapid detection of ofloxacin resistance in Mycobacterium tuberculosis by two low-cost colorimetric methods: resazurin and nitrate reductase assays

Anandi Martin et al. J Clin Microbiol. 2005 Apr.

Abstract

We have evaluated the performance of two rapid, low-cost methods for the detection of ofloxacin (OFX) resistance with 95 Mycobacterium tuberculosis isolates from countries with high multidrug-resistant tuberculosis endemicity. Results obtained by nitrate reductase and resazurin assays showed 100% agreement with those of the proportion method on 7H11 agar using 2 mug of OFX/ml. We confirmed the resistance of all isolates found to be resistant to OFX by the Mycobacterium Growth Indicator Tube system, and complete agreement among all methods was observed. Nitrate reductase and resazurin assays are rapid, simple, low-cost methods and might become inexpensive alternative procedures for rapid detection of OFX resistance in low-resource countries.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Dot plot diagram of the susceptibilities of 95 isolates of M. tuberculosis to OFX by the 7H11 agar proportion method versus the colorimetric MIC resazurin assay.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Δngeby, K. A., L. Klintz, and S. E. Hoffner. 2002. Rapid and inexpensive drug susceptibility testing of Mycobacterium tuberculosis with a nitrate reductase assay. J. Clin. Microbiol. 40:553-555. - PMC - PubMed
    1. Bastian, I., L. Rigouts, J. C. Palomino, and F. Portaels. 2001. Kanamycin susceptibility testing of Mycobacterium tuberculosis using Mycobacterium Growth Indicator Tube and a colorimetric method. Antimicrob. Agents Chemother. 45:1934-1936. - PMC - PubMed
    1. Berning, S. E. 2001. The role of fluoroquinolones in tuberculosis today. Drugs 61:9-10. - PubMed
    1. Bryskier, A., and J. Lowther. 2002. Fluoroquinolones and tuberculosis. Expert Opin. Investig. Drugs 11:233-258. - PubMed
    1. Canetti, G., F. Froman, J. Grosset, P. Hauduroy, M. Langerova, H. T. Mahler, G. Meissner, D. A. Mitchison, and L. Sula. 1963. Mycobacteria: laboratory methods for testing drug sensitivity and resistance. Bull. W. H. O. 29:565-578. - PMC - PubMed

Publication types

MeSH terms