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. 2005 Apr;43(4):1612-6.
doi: 10.1128/JCM.43.4.1612-1616.2005.

Rapid detection of ofloxacin resistance in Mycobacterium tuberculosis by two low-cost colorimetric methods: resazurin and nitrate reductase assays

Anandi Martin  1 , Juan Carlos Palomino, Françoise Portaels
Affiliations

Rapid detection of ofloxacin resistance in Mycobacterium tuberculosis by two low-cost colorimetric methods: resazurin and nitrate reductase assays

Anandi Martin et al. J Clin Microbiol. 2005 Apr.

Abstract

We have evaluated the performance of two rapid, low-cost methods for the detection of ofloxacin (OFX) resistance with 95 Mycobacterium tuberculosis isolates from countries with high multidrug-resistant tuberculosis endemicity. Results obtained by nitrate reductase and resazurin assays showed 100% agreement with those of the proportion method on 7H11 agar using 2 mug of OFX/ml. We confirmed the resistance of all isolates found to be resistant to OFX by the Mycobacterium Growth Indicator Tube system, and complete agreement among all methods was observed. Nitrate reductase and resazurin assays are rapid, simple, low-cost methods and might become inexpensive alternative procedures for rapid detection of OFX resistance in low-resource countries.

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Figures

FIG. 1.
FIG. 1.
Dot plot diagram of the susceptibilities of 95 isolates of M. tuberculosis to OFX by the 7H11 agar proportion method versus the colorimetric MIC resazurin assay.
See this image and copyright information in PMC

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