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. 2005 Apr;43(4):1921-4.
doi: 10.1128/JCM.43.4.1921-1924.2005.

Direct detection of Nocardia spp. in clinical samples by a rapid molecular method

Andrée Couble  1 , Verónica Rodríguez-Nava, Michèle Pérouse de Montclos, Patrick Boiron, Frédéric Laurent
Affiliations

Direct detection of Nocardia spp. in clinical samples by a rapid molecular method

Andrée Couble et al. J Clin Microbiol. 2005 Apr.

Abstract

We developed a 16S PCR-based assay for the rapid detection of Nocardia spp. directly from human clinical samples. The applicability of the assay was confirmed by using 18 samples from patients with nocardiosis as diagnosed by conventional cultures and 20 clinical samples from patients with confirmed tuberculosis used as negative controls.

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Figures

FIG. 1.
FIG. 1.
Analytical sensitivity of PCR protocol determined with clinical BAL sample seeded with Nocardia inocula of various sizes (shown as number of CFU per reaction mixture for each lane). (A) After electrophoresis in 1% agarose gel; (B) after Southern blot hybridization with a 16S probe. Lanes: M, molecular size markers (100-bp ladder); Neg, unseeded BAL sample (negative control).
FIG. 2.
FIG. 2.
Agarose gel electrophoresis and Southern blot hybridization of PCR products obtained from clinical samples from patients with confirmed nocardiosis. (A) β-Globin amplified products in 1% agarose gel; (B) Nocardia-specific 16S rRNA amplified products in 1% agarose gel; (C) Southern blot hybridization with chemiluminescent 16S probe. Lanes: M, molecular size markers (100-bp ladder); Neg, distilled water; 1 to 8, clinical samples.
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References

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