Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2005 Apr;43(4):1934-6.
doi: 10.1128/JCM.43.4.1934-1936.2005.

Comparison between rpoB and 16S rRNA gene sequencing for molecular identification of 168 clinical isolates of Corynebacterium

Atieh Khamis  1 , Didier Raoult, Bernard La Scola
Affiliations
Comparative Study

Comparison between rpoB and 16S rRNA gene sequencing for molecular identification of 168 clinical isolates of Corynebacterium

Atieh Khamis et al. J Clin Microbiol. 2005 Apr.

Abstract

Higher proportions (91%) of 168 corynebacterial isolates were positively identified by partial rpoB gene determination than by that based on 16S rRNA gene sequences. This method is thus a simple, molecular-analysis-based method for identification of corynebacteria, but it should be used in conjunction with other tests for definitive identification.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Dendrogram representing phylogenetic relationships of Corynebacterium type species and not-well-classified isolates by the neighbor-joining method. The tree was derived by alignment of concatenated 16S rRNA and rpoB genes sequences. The sizes of the 16S rRNA and rpoB gene sequences aligned ranged from 1224 to 1248 and 425 to 443 bp, respectively. The support of each branch, as determined from 1,000 bootstrap samples, is indicated by the value at each node (in percent). Only bootstrap values of ≥70% are given. Mycobacterium smegmatis was taken as the outgroup.
See this image and copyright information in PMC

References

    1. Daneshvar, M. I., D. G. Hollis, W. S. Weyant, J. G. Jordan, J. P. MacGregor, R. E. Morey, A. M. Whitney, D. J. Brenner, A. G. Steigerwalt, L. O. Helsel, P. M. Raney, J. B. Patel, P. N. Levett, and J. M. Brown. 2004. Identification of some charcoal-black-pigmented CDC fermentative coryneform group 4 isolates as Rothia dentocariosa and some as Corynebacterium aurimucosum: proposal of Rothia dentocariosa emend. Georg and Brown 1967, Corynebacterium aurimucosum emend. Yassin et al. 2002, and Corynebacterium nigricans Shukla et al. 2003 pro synon. Corynebacterium aurimucosum. J. Clin. Microbiol. 42:4189-4198. - PMC - PubMed
    1. Funke, G., and K. A. Bernard. 2003. Coryneform gram-positive rods, p. 472-501. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller, and R. H. Yolken (ed.), Manual of clinical microbiology. ASM Press, Washington, D.C.
    1. Funke, G., A. von Graevenitz, J. E. Clarridge III, and K. A. Bernard. 1997. Clinical microbiology of coryneform bacteria. Clin. Microbiol. Rev. 10:125-159. - PMC - PubMed
    1. Jackman, P. J. H., D. G. Pitcher, S. Pelczynska, and P. Borman. 1987. Classification of corynebacteria associated with endocarditis (group JK) as Corynebacterium jeikeium sp. nov. Syst. Appl. Microbiol. 9:83-90.
    1. Khamis, A., P. Colson, D. Raoult, and B. La Scola. 2003. Usefulness of rpoB gene sequencing for identification of Afipia and Bosea species including a strategy for the choice of discriminative partial sequences. Appl. Environ. Microbiol. 69:6740-6749. - PMC - PubMed

LinkOut - more resources