Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Apr;43(4):1940-3.
doi: 10.1128/JCM.43.4.1940-1943.2005.

Multiplex PCR for typing strains of Toxoplasma gondii

Daniel Ajzenberg  1 , Aurélien Dumètre, Marie-Laure Dardé
Affiliations

Multiplex PCR for typing strains of Toxoplasma gondii

Daniel Ajzenberg et al. J Clin Microbiol. 2005 Apr.

Abstract

A multiplex PCR assay was designed for multilocus strain typing of Toxoplasma gondii based on length polymorphism of five microsatellite markers. Eight T. gondii strains already sequenced at these five markers were used as control isolates. This method is simple, rapid, reproducible, and adapted to a large set of isolates.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Agarose gel electrophoresis of multiplex PCR amplification products with control isolates. Lanes: 1, BK; 2, ME49; 3, NED; 4, BOU; 5, DEG; 6, RMS-2001-MAU; 7, PSP-2003-KOM; 8, GUY-2002-MAT; V, molecular size markers.
FIG. 2.
FIG. 2.
Electrophoresis of multiplex PCR amplification products from a type II isolate (ME49) with an automatic sequencer by using GeneScan software. The x axis indicates a size fragment scale (in base pairs). The y axis indicates a peak height scale corresponding to the fluorescence intensity of detected peaks. Size fragments of GeneScan size standard ROX 500 are indicated between 75 and 500 bp.
See this image and copyright information in PMC

References

    1. Ajzenberg, D., N. Cogné, L. Paris, M. H. Bessieres, P. Thulliez, D. Filisetti, H. Pelloux, P. Marty, and M. L. Dardé. 2002. Genotype of 86 Toxoplasma gondii isolates associated with human congenital toxoplasmosis, and correlation with clinical findings. J. Infect. Dis. 186:684-689. - PubMed
    1. Ajzenberg, D., A. L. Bañuls, C. Su, A. Dumètre, M. Demar, B. Carme, and M. L. Dardé. 2004. Genetic diversity, clonality and sexuality in Toxoplasma gondii. Int. J. Parasitol. 34:1185-1196. - PubMed
    1. Burnett, A. J., S. G. Short, J. Isaac-Renton, A. King, D. Werker, and W. R. Bowie. 1998. Multiple cases of acquired toxoplasmosis retinitis presenting in an outbreak. Ophthalmology 105:1032-1037. - PubMed
    1. Carme, B., F. Bissuel, D. Ajzenberg, R. Bouyne, C. Aznar, M. Demar, S. Bichat, D. Louvel, A. M. Bourbigot, C. Peneau, P. Neron, and M. L. Dardé. 2002. Severe acquired toxoplasmosis in immunocompetent adult patients in French Guiana. J. Clin. Microbiol. 40:4037-4044. - PMC - PubMed
    1. Dardé, M. L., B. Bouteille, and M. Pestre-Alexandre. 1988. Isoenzymic characterization of seven isolates of Toxoplasma gondii by isoelectrofocusing in polyacrylamide gels. Am. J. Trop. Med. Hyg. 39:551-558. - PubMed

Publication types

LinkOut - more resources