Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Apr;43(4):1944-6.
doi: 10.1128/JCM.43.4.1944-1946.2005.

Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis

C F Albuquerque  1 , S H Marques da Silva, Z P Camargo
Affiliations

Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis

C F Albuquerque et al. J Clin Microbiol. 2005 Apr.

Abstract

In an attempt to improve the specificity of an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paracoccidioidomycosis (PCM), sera from patients with PCM were tested using various approaches, such as sodium metaperiodate antigen (gp43) treatment, a serum absorption process with Candida albicans or Histoplasma capsulatum antigens, and dilution of serum in galactose, the main common epitope among pathogenic fungi. The maximum specificity found in this ELISA was 84%. All of these procedures proved inefficient for eliminating all cross-reacting antibodies and obtaining an ELISA specific for PCM diagnosis.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Results of ELISAs using PCM and histoplasmosis sera, before and after absorption processes. A and B, sera from both groups of patients reacting with gp43, before and after treatment with 40 mM sodium metaperiodate; C, patient sera absorbed with C. albicans; D, patient sera absorbed with H. capsulatum; E, patient sera diluted with galactose; F, patient sera absorbed with C. albicans and H. capsulatum antigens and diluted in galactose.
See this image and copyright information in PMC

References

    1. Blotta, M. H. S. L., and Z. P. Camargo. 1993. Immunological response to cell-free antigens of Paracoccidioides brasiliensis: relationships with clinical forms of paracoccidioidomycosis. J. Clin. Microbiol. 31:671-676. - PMC - PubMed
    1. Camargo, Z. P., and M. F. Franco. 2000. Current knowledge on pathogenesis and immunodiagnosis of paracoccidioidomycosis. Rev. Iberoam. Micol. 17:41-48. - PubMed
    1. Camargo, Z. P., C. Unterkircher, S. P. Campoy, and L. R. Travassos. 1988. Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests. J. Clin. Microbiol. 26:2147-2151. - PMC - PubMed
    1. Camargo, Z. P., R. Berzaghi, C. C. Amaral, and S. H. Marques-da-Silva. 2004. Simplified method for producing Paracoccidioides brasiliensis exoantigens for use in immunodiffusion tests. Med. Mycol. 41:539-542. - PubMed
    1. Camargo, Z. P., J. L. Guesdon, E. Drouhet, and L. Improvisi. 1984. Enzyme-linked immunosorbent assay (ELISA) in the paracoccidioidomycosis. Comparison with counterimmunoelectrophoresis and erythro-immunoassay. Mycopathologia 81:31-37. - PubMed

Publication types

MeSH terms

LinkOut - more resources