doi: 10.1007/BF00019226.
Proton gradient-driven import of the 16 kDa oxygen-evolving complex protein as the full precursor protein by isolated thylakoids
Affiliations
- PMID: 1581565
- DOI: 10.1007/BF00019226
Item in Clipboard
Proton gradient-driven import of the 16 kDa oxygen-evolving complex protein as the full precursor protein by isolated thylakoids
Plant Mol Biol.
1992 Mar.
Abstract
The biogenesis of the lumenal 16 kDa protein of the photosynthetic oxygen-evolving complex was analysed using an assay for the import of proteins by isolated thylakoids. The precursor protein is imported with high efficiency in the light in both the presence and absence of stromal extract. Import is almost completely blocked in the dark or if the uncoupler nigericin is present in the light. The data indicate that transport across the thylakoid membrane is driven by a proton motive force in which the proton gradient is the dominant component, and that the full precursor protein can be transported across the thylakoid membrane without prior cleavage by the stromal processing peptidase.
Similar articles
-
A proton gradient is required for the transport of two lumenal oxygen-evolving proteins across the thylakoid membrane.J Biol Chem. 1991 Jul 5;266(19):12189-93. J Biol Chem. 1991. PMID: 1648086
-
Transport of proteins into chloroplasts. Requirements for the efficient import of two lumenal oxygen-evolving complex proteins into isolated thylakoids.J Biol Chem. 1991 Sep 15;266(26):17286-9. J Biol Chem. 1991. PMID: 1894619
-
Two distinct mechanisms for the translocation of proteins across the thylakoid membrane, one requiring the presence of a stromal protein factor and nucleotide triphosphates.J Biol Chem. 1994 Feb 4;269(5):3251-6. J Biol Chem. 1994. PMID: 8106361
-
Mechanisms of protein import into thylakoids of chloroplasts.Biol Chem. 2007 Sep;388(9):907-15. doi: 10.1515/BC.2007.111. Biol Chem. 2007. PMID: 17696774 Review.
-
Targeting of lumenal proteins across the thylakoid membrane.J Exp Bot. 2012 Feb;63(4):1689-98. doi: 10.1093/jxb/err444. Epub 2012 Jan 24. J Exp Bot. 2012. PMID: 22275386 Review.
Cited by
-
Arabidopsis mutants lacking the 43- and 54-kilodalton subunits of the chloroplast signal recognition particle have distinct phenotypes.Plant Physiol. 1999 Sep;121(1):61-70. doi: 10.1104/pp.121.1.61. Plant Physiol. 1999. PMID: 10482661 Free PMC article.
-
Maturation of thylakoid lumen proteins proceeds post-translationally through an intermediate in vivo.Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1862-6. doi: 10.1073/pnas.90.5.1862. Proc Natl Acad Sci U S A. 1993. PMID: 8446600 Free PMC article.
-
Degradation of mistargeted OEE33 in the chloroplast stroma.Plant Mol Biol. 1996 Mar;30(5):925-33. doi: 10.1007/BF00020804. Plant Mol Biol. 1996. PMID: 8639751
-
Transport of Folded Proteins by the Tat System.Protein J. 2019 Aug;38(4):377-388. doi: 10.1007/s10930-019-09859-y. Protein J. 2019. PMID: 31401776 Free PMC article. Review.
-
Evidence for a stromal GTP requirement for the integration of a chlorophyll a/b-binding polypeptide into thylakoid membranes.Plant Physiol. 1994 May;105(1):295-304. doi: 10.1104/pp.105.1.295. Plant Physiol. 1994. PMID: 8029355 Free PMC article.