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. 1974 Apr;5(4):362-5.
doi: 10.1128/AAC.5.4.362.

Screening method of agents against the R factor by the use of an Hfr made by integrative suppression with an R factor

M Yoshikawa

Screening method of agents against the R factor by the use of an Hfr made by integrative suppression with an R factor

M Yoshikawa. Antimicrob Agents Chemother. 1974 Apr.

Abstract

By the use of an Hfr strain made by integrative suppression of a derepressed R factor, R100-1 in a dnaA-ts mutant of Escherichia coli and several other appropriate control strains with and without the R factor, a screening system was established which can be used to detect agents which are either inhibitory to the replication of the R factor and lead to its "curing" or are preferentially inhibitory to the growth of the R-carrying bacteria. The validity of this system was shown with several agents already known to be experimentally effective.

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References

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