[Analysis of cytochrome c oxidase I and cytochrome b genes of F1 in laboratory line Oncomelania hupensis hupensis]
- PMID: 15830879
[Analysis of cytochrome c oxidase I and cytochrome b genes of F1 in laboratory line Oncomelania hupensis hupensis]
Abstract
Objective: To analyze the diversity of F1 in laboratory line Oncomelania hupensis hupensis.
Methods: Genomic DNA was isolated, and cytochrome c oxidase I (COI) and cytochrome b (Cytb) genes were amplified by polymerase chain reaction (PCR). The nucleotide sequences of the PCR products were analyzed by GENETYX-MAC software package (ver. 9).
Results: Pairwise divergences among six F1 individuals were found in 12.2% nucleotides in COI gene fragments. 13.5% of genetic divergences between O. h. hupensis and O. h. robertsoni were identified. 94 amino acids were observed in difference. In Cytb gene fragments, 6.4% of divergence was observed among four F1 samples. 25 amino acids were observed differently. The divergence of Cytb genes from O. h. robertsoni and O. h. hupensis were 13.6%, included 6 amino acids.
Conclusion: Diversities were found in both COI and Cytb gene sequences of F1 in laboratory line O. h. hupensis.
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