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. 2005 May;54(5):682-5.
doi: 10.1136/gut.2004.057281.

Hepatitis C virus replicates in peripheral blood mononuclear cells of patients with occult hepatitis C virus infection

I Castillo  1 , E Rodríguez-Iñigo, J Bartolomé, S de Lucas, N Ortíz-Movilla, J M López-Alcorocho, M Pardo, V Carreño
Affiliations

Hepatitis C virus replicates in peripheral blood mononuclear cells of patients with occult hepatitis C virus infection

I Castillo et al. Gut. 2005 May.

Abstract

Background: Occult hepatitis C virus (HCV) infection is characterised by the presence of HCV-RNA in the liver in the absence of anti-HCV, and serum viral RNA. Up to 70% of these patients also have HCV-RNA in peripheral blood mononuclear cells (PBMC) but it is not known if HCV is replicating in these cells.

Aim: We studied possible HCV replication in PBMC of 18 patients with an occult HCV infection who were selected on the basis of HCV-RNA positivity in PBMC.

Methods: Detection of HCV-RNA positive and negative strands in PBMC was done by strand specific reverse transcriptase-polymerase chain reaction (RT-PCR) and by in situ hybridisation.

Results: The presence of HCV-RNA positive strand in PBMC was confirmed in all patients by strand specific RT-PCR and by in situ hybridisation. Mean percentage of PBMC which had the HCV-RNA positive strand was 3.3% (95% confidence interval (CI) 2.1-4.4) The HCV-RNA negative strand was found in the PBMC of 11/18 (61%) patients by strand specific RT-PCR and confirmed by in situ hybridisation, and the percentage of PBMC harbouring the HCV-RNA negative strand was 3.1% (95% CI 0.8-5.5). There was a significant correlation (p = 0.001, r = 0.84) between the percentage of PBMC with the HCV-RNA positive strand and that of PBMC with the HCV-RNA negative strand.

Conclusion: HCV replicates in the PBMC of patients with occult HCV infection and thus, although these patients do not have serum HCV-RNA, they could be potentially infectious.

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Figures

Figure 1
Figure 1
Specificity and sensitivity analysis of the strand specific reverse transcriptase-polymerase chain reaction using synthetic hepatitis C virus (HCV)-RNA. (A) Positive strand RNA assay of HCV-RNA positive strand (0.1–100 fg) and HCV-RNA negative strand (1–100 pg). Lane N, negative control; lane M, 100 bp DNA ladder. (B) Negative strand RNA assay of HCV-RNA negative strand (0.1–100 fg) and HCV-RNA positive strand (1–100 pg). Lane N, negative control; lane M, 100 bp DNA ladder.
Figure 2
Figure 2
Correlation between percentages of peripheral blood mononuclear cells (PBMC) showing hybridisation signals for hepatitis C virus (HCV)-RNA positive and negative strands.
See this image and copyright information in PMC

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