Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 1992 May;29(5):299-307.
doi: 10.1136/jmg.29.5.299.

Reverse chromosome painting: a method for the rapid analysis of aberrant chromosomes in clinical cytogenetics

N P Carter  1 , M A Ferguson-Smith, M T Perryman, H Telenius, A H Pelmear, M A Leversha, M T Glancy, S L Wood, K Cook, H M Dyson, et al.
Affiliations
Case Reports

Reverse chromosome painting: a method for the rapid analysis of aberrant chromosomes in clinical cytogenetics

N P Carter et al. J Med Genet. 1992 May.

Abstract

We describe a method, termed reverse chromosome painting, which allows the rapid analysis of the content and breakpoints of aberrant chromosomes. The method involves the sorting of small numbers of the aberrant chromosome from short term blood culture preparations or cell lines by using bivariate flow karyotype analysis. The sorted chromosomes are amplified and biotin labelled enzymatically using a degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR), the product annealed to metaphase spreads from normal subjects, and hybridisation detected using fluorescence in situ hybridisation (FISH). We show the usefulness of this method for routine clinical cytogenetics by the analysis of cases involving an insertion, a deletion, a translocation, and two cases of a chromosome with additional material of unknown origin. The method has particular application for the rapid resolution of the origin of de novo unbalanced chromosome duplications.

PubMed Disclaimer

References

    1. Genes Chromosomes Cancer. 1992 Apr;4(3):257-63 - PubMed
    1. J Med Genet. 1991 Sep;28(9):577-82 - PubMed
    1. Prenat Diagn. 1990 Oct;10(10):677-81 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9664-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Dec;85(23):9138-42 - PubMed

Publication types

MeSH terms

LinkOut - more resources