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Comparative Study
. 1992 May 15;89(10):4514-8.
doi: 10.1073/pnas.89.10.4514.

Methods for the large-scale synthesis of psoralen furan-side monoadducts and diadducts

H P Spielmann  1 , S S Sastry, J E Hearst
Affiliations
Comparative Study

Methods for the large-scale synthesis of psoralen furan-side monoadducts and diadducts

H P Spielmann et al. Proc Natl Acad Sci U S A. .

Abstract

We report methods for the preparation of multimicromole quantities of extremely pure uniquely photo-adducted psoralen-DNA furan-side monoadducts and diadducts (cross-links). The methods use high-intensity krypton and argon ion lasers in the photoreactions and HPLC methods to purify the required oligonucleotides containing the photoadducts. With these methods we have synthesized 2-3 mumol of 8-mer psoralen furan-side monoadduct and diadduct. These methods allow one to generate large amounts of psoralenated DNA oligonucleotides and facilitate their study by NMR and x-ray crystallography.

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References

    1. J Mol Biol. 1991 Oct 20;221(4):1111-25 - PubMed
    1. Blood. 1989 Jul;74(1):517-25 - PubMed
    1. Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5602-6 - PubMed
    1. Nucleic Acids Res. 1991 Feb 11;19(3):657-63 - PubMed
    1. J Biol Chem. 1990 Oct 5;265(28):16898-912 - PubMed

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