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. 1992 May 1;256(5057):663-5.
doi: 10.1126/science.1585180.

Cloning and expression in yeast of a plant potassium ion transport system

H Sentenac  1 , N Bonneaud, M Minet, F Lacroute, J M Salmon, F Gaymard, C Grignon
Affiliations

Cloning and expression in yeast of a plant potassium ion transport system

H Sentenac et al. Science. .

Abstract

A membrane polypeptide involved in K+ transport in a higher plant was cloned by complementation of a yeast mutant defective in K+ uptake with a complementary DNA library from Arabidopsis thaliana. A 2.65-kilobase complementary DNA conferred ability to grow on media with K+ concentration in the micromolar range and to absorb K+ (or 86Rb+) at rates similar to those in wild-type yeast. The predicted amino acid sequence (838 amino acids) has three domains: a channel-forming region homologous to animal K+ channels, a cyclic nucleotide-binding site, and an ankyrin-like region.

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