Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 May;43(5):2449-51.
doi: 10.1128/JCM.43.5.2449-2451.2005.

Use of monoclonal antibodies to serotype Bordetella pertussis isolates: comparison of results obtained by indirect whole-cell enzyme-linked immunosorbent assay and bacterial microagglutination methods

Raymond S W Tsang  1 , Michelle L Sill, Abdolreza Advani, Dorothy Xing, Penny Newland, Hans Hallander
Affiliations

Use of monoclonal antibodies to serotype Bordetella pertussis isolates: comparison of results obtained by indirect whole-cell enzyme-linked immunosorbent assay and bacterial microagglutination methods

Raymond S W Tsang et al. J Clin Microbiol. 2005 May.

Abstract

Sixty-one Bordetella pertussis isolates were tested blindly in two laboratories to determine their serotype nature by monoclonal antibodies using two independent methods: the standard bacterial microagglutination assay and an indirect whole-cell enzyme-linked immunosorbent assay. Both methods gave concordant results in 60 of the 61 isolates.

PubMed Disclaimer

References

    1. Abdillahi, H., and J. T. Poolman. 1987. Whole-cell ELISA for typing Neisseria meningitidis with monoclonal antibodies. FEMS Microbiol. Lett. 48:367-371. - PubMed
    1. Advani, A., D. Donelly, and H. Hallander. 2004. Reference system for characterization of Bordetella pertussis pulsed-field gel electrophoresis profiles. J. Clin. Microbiol. 42:2890-2897. - PMC - PubMed
    1. Ashworth, L. A. E., L. I. Irons, and A. B. Dowsett. 1982. Antigenic relationship between serotype-specific agglutinogens and fimbriae of Bordetella pertussis. Infect. Immun. 37:1278-1281. - PMC - PubMed
    1. Blaskett, A. C., J. Gulasekharam, and L. C. Fulton. 1971. The occurrence of Bordetella pertussis serotypes in Australia, 1950-1970. Med. J. Aust. 1:781-784. - PubMed
    1. Brennan, M., P. Strebel, H. George, W. K. Yih, R. Tachdjian, S. M. Lett, P. Cassiday, G. Sanden, and M. Wharton. 2000. Evidence for transmission of pertussis in schools, Massachusetts 1996: epidemiological data supported by pulsed-field gel electrophoresis. J. Infect. Dis. 181:210-215. - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources