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. 2005 May 14;11(18):2709-13.
doi: 10.3748/wjg.v11.i18.2709.

Expression and clinical significance of TAp73alpha, p53, PCNA and apoptosis in hepatocellular carcinoma

Hai-Xia Qin  1 , Ke-Jun Nan, Guang Yang, Zhao Jing, Zhi-Ping Ruan, Chun-Li Li, Rui Xu, Hui Guo, Chen-Guang Sui, Yong-Chang Wei
Affiliations

Expression and clinical significance of TAp73alpha, p53, PCNA and apoptosis in hepatocellular carcinoma

Hai-Xia Qin et al. World J Gastroenterol. .

Abstract

Aim: To study the prognostic role of TAp73alpha, p53, proliferating cell nuclear antigen (PCNA) and apoptosis in patients with hepatocellular carcinoma (HCC) after surgical tumor ablation.

Methods: Forty-seven human resected HCC tissues and 42 adjacent non-cancerous tissues were studied with 10 normal liver tissues as control group. TAp73alpha, p53, and PCNA were detected with Elivision immunohistochemistry. Terminal deoxynucleotidyl transferase (TdT)-mediated d-UTP-biotin nick-end labeling (TUNEL) method was used to detect the apoptosis cells. All clinical and pathological materials were analyzed by SPSS10.0 statistical package.

Results: TAp73alpha overexpressed in HCC tissues (36.2%) when compared with adjacent non-cancerous tissues (2.38%, P<0.005) and normal liver tissues (0, P<0.01). Mutant type p53 (mt-p53) overexpressed in HCC tissues (38.3%) when contracted with adjacent non-cancerous tissues (16.7%, P<0.05) and normal liver tissues (0, P<0.01). Proliferation index (PI) level in HCC tissues was significantly higher than that in adjacent non-cancerous tissues (30.34%+/-4.46% vs 27.88%+/-5.89%, t, P = 0.028). Apoptosis index (AI) level in HCC tissues was higher than that in adjacent non-cancerous tissues (8.62%+/-2.28% vs 7.38%+/-2.61%, t, P = 0.019). Expression of TAp73alpha was associated with lymph node metastasis and mt-p53, with r = 0.407 and 0.265, respectively. Expression of mt-p53 was associated with Edmondson's stage and AFP, with r = 0.295 and -0.357, respectively. In Kaplan-Meier univariant analysis, TAp73alpha, AFP, TNM stage, portal vein invasion, liver membrane invasion and HBsAg correlated with prognosis (log rank, P = 0.039, 0.012, 0.002, 0.000, 0.014, 0.007, respectively). Multivariant Cox regression analysis showed that TAp73alpha, AFP, TNM stage, portal vein invasion, liver membrane invasion and age were independent factors of prognosis.

Conclusion: These results suggest that TAp73alpha can be used as a prognostic indicator of patients with HCC undergoing surgical tumor ablation. AFP, TNM, portal vein invasion, liver membrane invasion and age also have a potency of predicting the prognosis of HCC.

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Figures

Figure 1
Figure 1
TAp73α protein in HCC, nuclei of tumor cells were stained with yellow or brown (×400).
Figure 2
Figure 2
p53 protein in HCC, nuclei of tumor cells were stained with yellow or brown (×400).
Figure 3
Figure 3
PCNA protein in HCC, nuclei of tumor cells were stained with brown (×400).
Figure 4
Figure 4
Apoptosis cells were stained in blue, while non-apoptosis cells were stained in pink (×400).
See this image and copyright information in PMC

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