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. 2005 Mar-Apr;31(2):186-94.
doi: 10.1007/s11171-005-0023-7.

[A method for the preparation of normalized cDNA libraries enriched with full-length sequences]

[Article in Russian]
P A Zhulidov, E A Bogdanova, A S Shcheglov, I A Shagina, L L Vagner, G L Khazpekov, V V Kozhemiako, S A Luk'ianov, D A Shagin

[A method for the preparation of normalized cDNA libraries enriched with full-length sequences]

[Article in Russian]
P A Zhulidov et al. Bioorg Khim. 2005 Mar-Apr.

Abstract

We developed a new method for the preparation of normalized cDNA libraries enriched with full-length sequences. It is based on the properties of the recently characterized duplex-specific nuclease from the hepatopancreas of the Kamchatka crab. The duplex-specific nuclease is thermostable, it effectively cleaves double-stranded DNA and is inactive toward single-stranded DNA (Shagin et al., Genome Res., 2002, vol. 12, pp. 1935-1942). Our method enables the normalization of cDNA samples enriched with full-length sequences without use of laborious and ineffective stages of physical separation. The efficiency of the method was demonstrated in model experiments using cDNA samples from several human tissues.

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