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Review
. 2005 Apr;2(2):203-13.
doi: 10.1586/14789450.2.2.203.

Proteomics for biodefense applications: progress and opportunities

Richard R Drake  1 , Yuping Deng, E Ellen Schwegler, Stefan Gravenstein
Affiliations
Review

Proteomics for biodefense applications: progress and opportunities

Richard R Drake et al. Expert Rev Proteomics. 2005 Apr.

Abstract

The increasing threat of bioterrorism and continued emergence of new infectious diseases has driven a major resurgence in biomedical research efforts to develop improved treatments, diagnostics and vaccines, as well as increase the fundamental understanding of the host immune response to infectious agents. The availability of multiple mass spectrometry platforms combined with multidimensional separation technologies and microbial genomic databases provides an unprecedented opportunity to develop these much needed resources. An overview of current proteomic strategies applied to microbes and viruses considered potential bioterrorism agents is presented. The emerging area of immunoproteomics as applied to the development of new vaccine targets is also summarized. These powerful research approaches can generate a multitude of potential new protein targets; however, translating these proteomic discoveries to useful counter-bioterrorism products will require large collaborative research efforts across multiple basic science and clinical disciplines. A translational proteomic research paradigm illustrating this approach using influenza virus as an example is discussed.

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Figures

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IMAC-Cu: Copper-coated immobilized metal ion affinity chromatograph; SAX: Strong anion exchange; SELDI: Surface-enhanced laser desorption/ionization; WCX: Weak cation exchange.
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IMAC-Cu: Copper-coated immobilized metal ion affinity chromatograph; MW: Molecular weight; PLUNC: Palate, lung and nasal epithelial clone; SELDI: Surface-enhanced laser desorption/ionization.
See this image and copyright information in PMC

References

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    2. Illustrates the ability of proteomics to generate comprehensive and comparative subproteome information.

    1. Elhanany E, Barak R, Fisher M, Kobiler D, Altboum Z. Detection of specific Bacillus anthracis spore biomarkers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom. 15(22), 2110–2116 (2001). - PubMed
Websites
    1. NIAID Biodefense Research www2.niaid.nih.gov/biodefense/research/ strat_plan.htm (Viewed March 2005)
    1. NIAID Category A, B and C Priority Pathogens www2.niaid.nih.gov/Biodefense/bandc_ priority.htm (Viewed March 2005)

MeSH terms

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